Table 2.
Description of alpiniamide biosynthesis gene cluster and deduced function of alp-genes.
| ID | Name | Predicted function | Closest characterized homologs (% of identical amino acids) | GenBank accession number |
|---|---|---|---|---|
| 12715 | alpI | fatty acyl-CoA reductase | putative short chain dehydrogenase, hitachimycin, 46% (Streptomyces scabrisporus) | LC008143 |
| 12720 | alpH | TetR family transcriptional regulator | ∗TetR/AcrR family; Streptomyces sp. IB2014 011-1 (100%) | - |
| 12725 | alpW | transporter, ATP binding protein | UvrA-like protein, quinomycin, 76% (Streptomyces griseovariabilis) | JN852959 |
| 12730 | alpG | glyoxalase protein | putative lyase, pentalenolactone, 82% (Streptomyces avermitilis) | BA000030 |
| 12735 | alpF | transcriptional activator/DNA repair enzyme | putative AraC-family transcriptional regulator, pentalenolactone, 86% (Streptomyces avermitilis) | BA000030 |
| 12740 | alpT | malonyl-CoA ACP transacylase | acyltransferase/oxidoreductase, chivosazole, 50% (Sorangium cellulosum) | DQ065771 |
| 12745 | alpE | 3-oxoacyl-ACP synthase 3 | 3-oxoacyl-ACP synthase, cosmomycin D, 48% (Streptomyces olindensis) | JJOHO1000002 |
| 12750 | alpA3 | PKS: KS, AT, ACP; KS, DH, KR, ACP | polyketide synthase, kijamimicin 48% (Actinomadura kijaniata) | EU301739 |
| 12755 | alpA2 | PKS: KS, ACP, cMT, TE | polyketide synthase, bacillaene, 38% (Bacillus amyloliquefaciens) | AJ634060 |
| 12760 | alpA1 | PKS/NRPS: C, A, PCP; KS, ACP, cMT; KS, DH, tDH, KR, ACP | OnnI, Onnamide, 44% (Candidatus Entotheonella sp.) | AY688304 |
| 12765 | alpD | acyl-CoA dehydrogenase | ∗acyl-CoA dehydrogenase, dutomycin, 88% (Streptomyces minoensis) | KP710956 |
| 12770 | alpR | ribosomal RNA large subunit methyltransferase G | methyltransferase containing protein; Streptomyces sp. IB2014 011-1 (100%) | - |
∗shown are closest homologs.