Figure 6.

P2-16-mer pretreated LN cells did not proliferate after stimulation with P2 peptides. Twelve days after immunization with P2_53–78 peptide, LN cells from immunized animals were exposed to 10 μg of P2_53–78 peptide, P2-16-mer, or no antigen (first stimulation). LN cells were propagated by using IL-2-containing medium and were challenged by using 10 μg of the indicated antigens on day 8 (second stimulation). The specific proliferative response was measured by [³H]thymidine incorporation. Note that the y axis is interrupted for the proliferative response of cells stimulated with P2_53–78 peptide in both cycles. Results are expressed as mean cpm + SD of triplicates.