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. 2018 Aug 22;9:478. doi: 10.3389/fendo.2018.00478

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Copyright © 2018 Bendix, Laursen, Mortensen, Melikian, Globa, Detlefsen, Rasmussen, Petersen, Brusgaard and Christesen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histology of ectopic pancreatic tissue located in the wall of the duodenum, revealing features of focal CHI. The tissue section has been used for intraoperative frozen section analysis prior to fixation and paraffin embedding. (A) Focal adenomatous hyperplasia, showing endocrine cells involving more than 40% of the area (H&E staining). (B) The endocrine nature of the cells is emphasized by immunohistochemistry (synaptophysin immunostaining). (C) In the upper right corner, the duodenal surface with mucosa containing a few Brunner's glands is shown (Alcian blue periodic acid-Schiff staining). (D) Strong expression of CDX-2 in the duodenal mucosa (CDX2 immunostaining).