Table 1.
Oligonucleotide primers used for generation of mutant libraries for affinity maturation
| Name | Primer 5′‐3′ |
|---|---|
| L1‐F | AATCAGACCGATAAACTGGCG |
| L1‐Rb | GCCAGTTTATCGGTCTGATTMNNCGGGCTMNNACGMNNCCAMNNCAGMNNAAACGATTCGCTC |
| L2‐Fb | GGTATCGTATGAGCCCGTCTNNKNNKNNKGATNNKCTGGCGNNKTTCCCGGAAGATCGCTCTCAGCC |
| L2‐R | GACGGGCTCATACGATACCAGTTCAG |
| L3‐F1b | CAGACCGATAAACTGGCGNNKTTCNNKGAAGATCGCTCTCAGNNKNNKCAAGACTGCCGTTTTCGC |
| L3‐R1 | CGCCAGTTTATCGGTCTGATTAG |
| L3‐F2b | CGCAATGACTCCGGCACCTACNNKTGTGGTGCAATTTCACTGGC |
| L3‐R2 | GTAGGTGCCGGAGTCATTGCG |
| L4‐Fb | CTCCGGCACCTACCTGTGTNNKNNKNNKTCANNKGCTNNKAAAGCCCAAATCAAAGAATCGC |
| L4‐R | ACACAGGTAGGTGCCGGAGTC |
| L5‐Fb | GGTGCAATTTCACTGGCTCCGNNKNNKNNKNNKAAANNKTCGCTGCGTGCGGAACTGCG |
| L5‐R | GAGCCAGTGAAATTGCACCACACAGG |
| Primer‐Fa | CTCATGCCATGGCAAATCCGCCGACG |
| Primer‐Ra | AAGGAAAAAAGCGGCCGCTTCGGCACGACGTTC |
a
NcoI/NotI are indicated by italic sequences.
b
In some of the primers, K was replaced with a mixture of G/T; M was replaced with a mixture of C/A; N was replaced with a mixture of A/T/C/G. The frequency of each base in each mixture was equal.