Figure 8.

Flavokawain (FKB) induces cytoprotective autophagy in thyroid cancer cells both in vitro and in vivo. (A,B) WRO cells were pretreated with 3‐methyladenine (3‐MA) (10 mmol/L) or si‐Beclin‐1, then incubated with 6 μg/mL FKB or DMSO for another 24 h. Cell viability was detected using CCK‐8 assays. (C–F) Mice were killed 18 days after the indicated treatments, and tumor weight, tumor volume, and mouse body weight were measured. (G) TUNEL assay and immunohistochemical staining results of Ki67 on tumor sections. All data are expressed as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001