Sensitivity of the LAMPAuris approach. The reaction was run at 56°C for 90 min; the sensitivity of 2 × 101 copies per reaction was confirmed in triplicate reactions. A, negative control (no reaction); B, 2 × 100 copies/reaction (34 min); C, 2 × 101 copies/reaction (33 min); D, 2 × 102 copies/reaction (26 min); E, 2 × 103 copies/reaction (24 min); F, 2 × 104 copies/reaction (24 min); G, 2 × 106 copies/reaction (22 min); H, 2 × 108 copies/reaction (17 min).