TABLE 2.

Establishment of the ΔC_T^a for each AMR reaction based on the results for the Neisseria gonorrhoeae control isolates

Target	No. of wild-type strains	ΔCT range for wild-type strainsb	No. of mutant strains	ΔCT range for mutant strains	Established ΔCT for positivity
penA Gly545Ser	37	15.65 to >25.8	8	0.21 to 3.17	4
penA Asp345del	35c	9.65 to >25.25	10	−0.86 to 2.64	4
23S rRNA C2611T	43	7.60 to 12.84	2	−0.12 to −1.82	1
23S rRNA A2059G	43	9.35 to 15.20	2	0.73 to 0.76	2
gyrA Ser91Phe	15	13.92 to 16.93	30	1.01 to 5.78	7
16S rRNA C1192T	43	17.07 to 24.12	2	6.67 to 9.35	10

^aΔC_T, difference between the C_T of the AMR reaction and the C_T of the opa reaction.

^bWhen the C_T was >40, the upper range could not be estimated precisely.

^cResults for strains WHO X and WHO Z, carrying a rare mosaic penA allele, were considered negative and included in the range for wild-type isolates.