Figure 4.

Caveolin (CAV) knockdown affects hepatocellular carcinoma cell proliferation and triglyceride (TG) accumulation. (A,B) Quantitative RT‐PCR (A) and Western blot (B) analyses of cells transfected with siRNAs against CAV‐1 or CAV‐2 or with a non‐targeting (NT) siRNA. (C) Proliferation of HLE cells cultured in the presence of oleic acid (OA) after siRNA transfection (48 h). The ratio was determined as the number of cells cultured in DMEM + 3% BSA + 500 μmol/L OA relative to the number of cells cultured in DMEM + 3% BSA. **P < 0.01, Student's t‐test and Mann–Whitney U‐test. (D) Liquid chromatography–tandem mass spectrometry analysis of intracellular TG level in HLE cells cultured in medium containing 500 μmol/L OA after CAV‐1 or CAV‐2 knockdown. (E) TG/diacylglycerol (DG) ratio. *P < 0.05, Student's t‐test and Mann–Whitney U‐test. Results are expressed as the mean ± SD