FIG 5.
Elevated expression of HSF1 targets in tissues of HSF1303A/307A mice maintained on a normal diet. WT and HSF1303A/307A mice were untreated or exposed to whole-body hyperthermia at 41.5°C for 40 min and allowed to recover for 6 h. Protein extracts from different tissues were assessed by immunoblotting with antibodies specific for HSP70 and HSPB1. Quantification of the HSP protein levels (normalized by WT mice) is shown (n = 3 mice for each genotype). For all panels, bars are means ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Red, WT mice; blue, HSF1303A/307A mice.