FIG 7.

The lack of S303/S307 phosphorylation of HSF1 impacts metabolic mouse phenotype under normal diet but not HFD feeding conditions The metabolic activity of 5-month-old HSF1^303A/307A and WT mice maintained on an ND (A) or exposed to a short period of 2 weeks on HFD (B) was determined using a calorimetric caging system. The animals were acclimatized for 3 days at 25°C before data were recorded for 4 light/dark cycles. Food intake, O₂ consumption, CO₂ production, and ambulatory activity of the mice were recorded every 2 min. The respiratory exchange rate (RER) (CO₂/O₂ ratio) and rate of energy expenditure (heat) were calculated. Graphs show quantification of metabolic parameters. Error bars are means ± SD (n = 6 mice for each genotype). ***, P < 0.001. Red, WT mice; blue, HSF1^303A/307A mice. The corresponding Zeitgeber hours are indicated at the bottom (0 is the time at which light is on).