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. 2001 Nov 20;98(24):13884–13888. doi: 10.1073/pnas.241358198

Figure 1.

Figure 1

Reconstitution of DR-mediated apoptosis in I9.2 Jurkat cells. I9.2 cells were transfected with 12 μg of vector or various constructs and 4 μg of a GFP construct. Caspase-8 (casp8), short (casp10S) and long (casp10L) isoforms of caspase-10, caspase-3 (casp3), and mutants (mu) of caspase-8 and -10 containing mutated protease-active sites were used. Cells were treated 8 h later with FasL (A) or TRAIL (B) for 24 h, and the percentage cell loss of GFP+ cells that are negative for annexin V was quantitated (13). Transfected cells positive for annexin V staining without FasL or TRAIL treatment are below 5% (data not shown).