FIG 2 .

Calcium regulates brp expression and biofilm formation by increasing intracellular c-di-GMP levels. (A) V. vulnificus biofilm formation on coverslips during growth in LB or LB supplemented with 50 mM MgCl₂ (LB^Mg) or 10 mM CaCl₂ (LB^Ca). (B) LC-MS/MS quantification of c-di-GMP from whole-cell extract of V. vulnificus grown in the same medium. Plots show the mean, and error bars represent the standard deviation. Statistically significant differences among the samples (P < 0.001 as determined by one-way ANOVA followed by pairwise comparisons with a Bonferroni adjustment) are indicated by different symbols above each bar.