FIG 6 .

Mutations in cysC and cysD inhibit rugose colony formation in response to elevated intracellular c-di-GMP levels and calcium-induced pellicle formation. (A) Rugose colony development by the indicated strains under unaltered (v [empty vector]) or elevated (DcpA) intracellular c-di-GMP levels on LB agar plates supplemented with 0.5 mM cysteine (LB^cys). (B) Quantification of the surface area of the rugose colonies in panel A. (C) The wild-type (WT) and cysD mutant strains were grown in LB^cys or LB^cys+Ca, and pellicle formation at the air-liquid interface was imaged. The left image in each panel is the top-down view of the pellicle, and to the right is the 3D rendering, where the dark-to-light transition indicates low-lying to elevated surface area regions. The scale is to the right in each panel. (D) Quantification of the pellicle surface area in panel C.