FIG 3 .

Brefeldin A (BFA) attenuates secretion of INF4 and pectinesterase (PE1) from haustoria in planta. Confocal projections of the same transformants used in Fig. 2 in infected Nicotiana benthamiana revealed that INF4-mRFP and PE1-mRFP were secreted at haustoria. After BFA treatment, INF4-mRFP and PE1-mRFP were retained in the hyphae and haustoria, accumulating in vesicle-like structures, rather than outlining haustoria as in the water-treated control. White arrows represent the lines used for the fluorescence intensity profiles shown in the graphs to the right of each set of images. The x-axis data in the graphs represent the distances (in micrometers) from one end of each white arrow in the images to the other end. Scale bars = 10 µm. The BFA panel was imaged after 24 h of exposure to BFA (50 µg/ml). These images are representative of three independent biological replicates.