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. 2018 Jul 8;141(9):2700–2710. doi: 10.1093/brain/awy183

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© The Author(s) (2018). Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Prion seeding activity in scrapie-exposed PrP transgenic Drosophila. Elav × VRQ(GPI) PrP transgenic (VRQ) and Elav × 51D (51D) Drosophila were exposed at the larval stage to PG127 scrapie-infected or prion-free control sheep brain material. At various times after hatching, head homogenate was prepared from harvested flies and used as seed in PMCA reactions. (A) End-point titration of PMCA prion seeding activity in head homogenate from control or PG127-exposed Drosophila. (B) Western blot detection of PK-resistant PrP27–30 in PMCA reaction products seeded with control or PG127-exposed Drosophila head homogenate. Molecular mass markers in kDa shown on the left.