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. 2018 Aug 22;9:884. doi: 10.3389/fphar.2018.00884

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Copyright © 2018 Du, Wang, Liu, Wang, Su, Zhang and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the abundance of SCMs generated from HILIC column chromatography in AG, RG, and HC groups in Validation phase. (A), 2-Hexenoylcarnitine; (B),1-Methylhistidine; (C), Asymmetric dimethylarginine; (D), 5′-Methylthioadenosine; (E), Butyrylcarnitine; (F), N-Acetylputrescine; (G), Creatinine; (H), Valerylcarnitine; (I), 1-Methyladenosine. *indicates P < 0.05, **indicates P < 0.01, and ***indicates P < 0.001. P < 0.05 indicates statistical significance. SCMs, significantly changed metabolites. Targeted metabolomics analyses was used to validate the abundance of SCMs in Validation phase. The HC, RG, and AG groups in Validation phase included 20, 26, 29 subjects, respectively.