Figure 1.

FBCs Actively Migrate toward Epithelial Wounds

(A) Images of pupae and methylene blue-stained section of the pupal thorax (FBCs false-colored green) showing FBC location and indicating site of laser wounding in the ventral thorax (blue arrows).

(B and C) Schematic (B) and time-lapse (C) images to illustrate FBC migration to a wound (projection, C top; Z plane, B and C bottom) in a c564-Gal4+UAS-GFP+Ubq>Histone-RFP pupa (epithelial nuclei in red; FBCs in green and outlined; asterisk labels wound-associated FBCs; arrowheads indicate wound margins). See also Movie S2, first movie, and Figure S1.

(D–H) Time lapse (D–F) and graphs (G and H) showing how FBCs are drawn to small, medium, and large wounds (30–60 μm, 60–90 μm, and 90–120 μm in diameter; n = 32, 12, and 15, respectively) in c564-Gal4+UAS-GFP+Ubq>Histone-RFP pupae (epithelial nuclei in red; FBCs in green and outlined). See also Movie S2, second, third, and fourth movies.

(I) Graph showing duration of reepithelialization (pink bar) and FBC presence (yellow bar) in small, medium, and large wounds (n = 17, 11, and 11, respectively; genotype as in D–H). Mean ± SEM.

Scale bars, 20 μm (C and D). (E) and (F) are the same magnification as (D).