Figure 3.

FBCs Actively Migrate toward Wounds Using a Novel Actomyosin-Driven Peristaltic Mode of Motility

(A–C) Time lapse (projection at top, single plane beneath) showing actin dynamics in FBCs within unwounded (A) or wounded (C) Lpp-Gal4+UAS-GMA pupae (GMA shown in ImageJ LUT Fire). Location of the wound indicated by white dashed circle. Schematic illustrating peristaltic migration (B). See also Movie S3.

(D–F) Low-magnification (D) and high-magnification (E and F) images of Lpp-Gal4+UAS-rd-Tomato+control or +UAS-DN-Zip-YFP pupae; FBCs in red, DN-Zip-YFP in yellow; asterisk marks absence of FBCs in head (D); 1-hr migration tracks of FBCs, white lines (F). See also Movie S6 and Figure S4. Control = w67.

Scale bars, 20 μm (A, C, E, and F) and 200 μm (D).