REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E.coli: RFP-E.coli	Vlisidou et al., 2009	N/A
Experimental Models: Organisms/Strains
D. melanogaster: w67	Gift from Jordan Raff	N/A
D. melanogaster: Lpp-Gal4	Gift from Pierre Leopold	N/A
D. melanogaster: c564-Gal4	Kambris et al., 2006	N/A
D. melanogaster: srp-Gal4	Brückner et al., 2004	N/A
D. melanogaster: tub-Gal80ts	Bloomington Drosophila stock center	RRID: BDSC7017
D. melanogaster: UAS-GFP-Ena	Gates et al., 2007	N/A
D. melanogaster: UAS-Cherry-Fimbrin	Gift from Tom Millard	N/A
D. melanogaster: UAS-GFP-Fascin	Zanet et al., 2009	N/A
D. melanogaster: UAS-GFP	Bloomington Drosophila stock center	RRID: BDSC_6874
D. melanogaster: UAS-GFP	Bloomington Drosophila stock center	RRID: BDSC_6658
D. melanogaster: UAS-myr-td-Tomato	Bloomington Drosophila stock center	RRID: BDSC_32221
D. melanogaster: UAS-rd-Tomato	Bloomington Drosophila stock center	RRID: BDSC_36327
D. melanogaster: UAS-nuclear-Red-Stinger	Barolo et al., 2004	N/A
D. melanogaster: UAS-GMA	Dutta et al., 2002	N/A
D. melanogaster: UAS-DN-Zipper-YFP	Dawes-Hoang et al., 2005	N/A
D. melanogaster: UAS-Reaper	Bloomington Drosophila stock center	RRID: BDSC_5824
D. melanogaster: srp>3xmCherry	Gyoergy et al., 2018	N/A
D. melanogaster: srp-GMA	Moreira et al., 2010	N/A
D. melanogaster: sqh>Sqh-GFP	Royou et al., 2004	N/A
D. melanogaster: Ubq>Hist-RFP	Bloomington Drosophila stock center	RRID: BDSC_23651
D. melanogaster: Ubq>RFP-α-tubulin	Basto et al., 2008	N/A
D. melanogaster: Ubq>GFP-α-tubulin	Basto et al., 2008	N/A
D. melanogaster: Attacin>GFP	Tzou et al., 2000	N/A
Software and Algorithms
Volocity	PerkinElmer	http://cellularimaging.perkinelmer.com/downloads/
ImageJ/Fiji	Fiji	http://fiji.sc/
Photoshop	Adobe	http://www.adobe.com/uk/products/photoshop.html
Illustrator	Adobe	http://www.adobe.com/uk/products/illustrator.html
Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/
Excel	Microsoft	https://www.microsoft.com/en-gb/
Other
Glass bottom microscopy dish	Greiner Bio-One GmbH	627861
Multi-laser CLSM confocal microscope (Leica SP5II and SP8)	Leica	http://www.leica-microsystems.com/home/
63x NA1.4 Plan-Apochromat oil objective	Leica	http://www.leica-microsystems.com/home/
Zeiss Lightsheet Z.1 microscope	Carl Zeiss	https://www.zeiss.com/corporate/int/home.html
Perkin Elmer UltraView spinning disc system	PerkinElmer	https://science.nichd.nih.gov/confluence/display/mic/Perkin-Elmer+Ultraview+RS
Transmission electron microscope Tecnai 12-FEI 120kV BioTwin Spirit	Tecnai (Thermo Fisher Scientific)	https://www.fei.com/tecnai-upgrades/