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. 2018 Aug 22;6:342. doi: 10.3389/fchem.2018.00342

Figure 5.

Figure 5

Overlay of extracted ion chromatograms (EIC) for chromatographic separation of steryl glucosides (SG) from standards or extracted from foods detected as [SG+Na]+ on a C18 UPLC column applying a methanol/water gradient with 0.1% formic acid as additive; isomeric compounds have the same color and are distinguished by different line patterns; (A) EIC of all analyzed SG in one plot; (B) EIC of SG with m/z 571 - m/z 587; (C) EIC of SG with m/z 595 - m/z 597, and (D) EIC of SG with m/z 599 - m/z 611; (a) poriferasta-7,22,25-dienyl glucoside (m/z 595) from pumpkin seeds; (b) 24-methylenecholesteryl glucoside (m/z 583) from rapeseeds; (c) poriferasta-7,25-dienyl glucoside (m/z 597) from pumpkin seeds; (d) Δ7-avenasteryl glucoside (m/z 597) from SG standard mixture; (e) brassicasteryl glucoside (m/z 583) from rapeseeds; (f) cholesteryl glucoside (m/z 571) from a standard; (g) Δ5-avenasteryl glucoside (m/z 597) from oat bran; (h) Δ7-campesteryl glucoside (m/z 585) from melon; (i) spinasteryl glucoside (m/z 597) from pumpkin seeds; (j) stigmasteryl glucoside (m/z 597) from SG standard mixture; (k) campesteryl glucoside (m/z 585) from SG standard mixture; (l) cholestanyl glucoside (m/z 573) from potato peel; (m) citrostadienyl glucoside (m/z 611) from potato peel; (n) Δ7-stigmastenyl glucoside (m/z 599) from melon; (o) sitosteryl glucoside (m/z 599) from SG standard mixture; (p) campestanyl glucoside (m/z 587) from wheat bran and (q) sitostanyl glucoside (m/z 601) from wheat bran. Accurate m/z are listed in Table 1 and peaks were extracted from chromatograms with a mass window of 0.01 Da. Only peaks of interest were isolated.