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Relative retention times (RRT) of free sterols (FS) on a C18 UPLC column with a methanol/water gradient.
| FS | RRT to cholesterol |
|---|---|
| Desmosterol | 0.95 |
| Ergosterol | 0.96 |
| 7-Dehydrocholesterol | 0.97 |
| 24-Methylenecholesterol | 0.97 |
| Brassicasterol | 0.99 |
| Cholesterol | 1.00 |
| Δ5-Avenasterola | 1.00 |
| Lanosterol | 1.01 |
| Coprostanol | 1.01 |
| Spinasterol | 1.02 |
| Cholestanol | 1.02 |
| Campesterol | 1.02 |
| Stigmasterol | 1.02 |
| Sitosterol | 1.05 |
| Sitostanol | 1.07 |
Not obtained as pure standard compound but extracted from oat bran.
