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. 2018 Aug 22;9:661. doi: 10.3389/fneur.2018.00661

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Copyright © 2018 Chiu, Lin, Tzang, Li, Lin, Das, Chen, Chen and Hsu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double labeling with the mouse anti-NR1 mAb confirmed test results for clinical CSF samples. The left images were single-labeling results for the two CSF samples. The right images were from the two double-stain protocols (as in Figures 3B,C). TOP: CSF test results from Patient A. BOTTOM: CSF test results from patient B. Yellow arrowheads pointed to sites of colocalization of green and red fluorescence (overlay in yellow-orange color). Scale bars, 20 μm.