Figure 4.

Hub cells dictate whole-islet Ca²⁺ activity in a model of a human islet. (A) 3D plot of ${\left[C{a}^{2+}\right]}_i$ for each β-cell in a human islet model, during hub inhibition and non-hub inhibition. cf. S3 Video. (B) Mean ${\left[C{a}^{2+}\right]}_i$ for all β-cells in a human islet model, during hub inhibition and non-hub inhibition. Raster plot showing ${\left[C{a}^{2+}\right]}_i$ activity in each β-cell during the hub inhibition condition. (C) ${\left[C{a}^{2+}\right]}_i$ activity in a human islet model as a function of the number of cells inhibited (% of islet). Either hubs or non-hubs were inhibited. Error bars show the SEM for re-running of both of these simulations for 6 different random seeds. (D) Comparison of the IC₅₀ of (C) in the human islet and mouse islet. Represented as % of hubs (which is 10% of the islet). Unpaired t-test, ** = p < 0.01.