Figure 4.

VHL Contributes to Surfactant Handling by AMs

(A) OCR by BAL AMs in the presence of palmitoyl-CoA treated as seen in Figure 2D. Data are means ± SDs and are representative of three independent experiments. n = 6–7 mice of each genotype.

(B and C) Basal respiratory rate (BRR) (B) and basal ECAR (C) of BAL AMs. Data represent three independent experiments. n = 6–7 mice per genotype. ^∗p < 0.05; ns, not significant, by ratio paired Student’s t test.

(D) Protein concentration in BAL from 3-month-old mice. Data are means ± SEMs and representative of three independent experiments. n = 5–9 mice per genotype. ns, not significant, by unpaired Student’s t test.

(E–G) 5 × 10⁴ AMs from control Vhl^fl/fl or CD11cΔVhl AMs were transferred intratracheally (i.t.) to Csfr2b^−/− mice. Scheme of the experiment (E). After 1.5 months, lung AM numbers (F) and BAL concentrations of total protein (G [left]) and SP-D (G [right]) were determined. Data represented as means ± SEMs of three pooled independent experiments. Additional control mice (Csfr2b^−/− and Csfr2b^+/−) were added. n = 10–18 per group. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; ns, not significant, by one-way ANOVA and Bonferroni post hoc test. Only some relevant comparisons indicated.

See also Figure S2.