Figure 3.

Nur77-Deficient Macrophages Fail to Downregulate IDH Expression and Accumulate Higher Levels of Succinate during Inflammation

(A) Hierarchical clustering and expression heatmap of TCA cycle enzyme-encoding genes in RAW264.7 after inducible overexpression of GFP or HA-NUR77, as determined by RNA-seq.

(B) Mean Nur77 ChIP-seq signal in RAW264.7 in 20 kb window around transcription start sites (TSSs) of TCA cycle genes shown in (A).

(C) Genome browser view of Nur77 ChIP-seq signal around isocitrate dehydrogenase (IDH) gene loci in RAW264.7 macrophages.

(D and E) Gene expression of cytosolic and mitochondrial IDH isoforms (D) and succinate levels (E) in WT and Nur77-KO BMDMs left untreated or stimulated with LPS for the times indicated.

For (D) and (E), data are shown as means ± SEMs (n = 3). p values were calculated using two-tailed Student’s t test. n.s., non-significant. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

See also Figure S3.