Table 1.
Primer sequences and thermal profiles
| Genes | Sequence (5’to 3’) | Amplification Size(bp) |
|---|---|---|
| GAPDH | F: GGAAGGTGAAGGTCGGAGTCA | 101 bp |
| R:GTCATTGATGGCAACAATATCCACT | ||
| TWIST1 | F: GGAGTCCGCAGTCTTACGAG | 201 bp |
| R:TCTGGAGGACCTGGTAGAGG | ||
| BMI1 | F: CGTGTATTGTTCGTTACCTGGAGAC | 204 bp |
| R:CATTGGCAGCATCAGCAGAAGG | ||
| Cripto | F: GGGATACAGCACAGTAAGGAG | 295 bp |
| R:ACGGTGGTAGTTGTCGAGTC | ||
| KLF4 | F:TCTTCTCTTCGTTGACTTTG | 210 bp |
| R:GCCAGCGGTTATTCGG | ||
| Nanog | F: GGCAATGGTGTGACGCAGAAGGC | 137 bp |
| R:GCTCCAGGTTGAATTGTTCCAGGTC | ||
| MSI1 | F: TGAGCAGTTTGGGAAGGTG | 117 bp |
| R: TCACACACTTTCTCCACGATG | ||
| MEIS1 | F: ATGACACGGCATCTACTCGTTC | 105 bp |
| R: TGTCCAAGCCATCACCTTGCT | ||
| SOX2 | F: AACAGCCCGGACCGCGTCAA | 189 bp |
| R: TCGCAGCCGCTTAGCCTCGT | ||
| MAML1 | F: TCTCGCGGAACAGGAGA | 123 bp |
| R: GCAGCAGAGGACCCTGTG | ||
| PYGO2 | F: GTCCCCCACTCCATGGCCGCCTCG | 147 bp |
| R: TCGCTTCTTTTCTGGACTCTTC | ||
| EGFR | F: ACCGGCAGGATGTGGAGATC | 186 bp |
| R: GGCCGACAGCTATGAGATGGA | ||
| ABCG2 | F: TGAGGGTTTGGAACTGTGG | 155 bp |
| R: GATTCTGACGCACACCTGG | ||
| ABCC4 | F: GAAATTGGACTTCACGATTTAAGG | 125 bp |
| R: TTCCACAGTTCCTCATCCGT | ||
| DIDO1 | F: TTTGTTGGTCCAGTTTCGCCTTC | 220 bp |
| R: ACGACAAGCAAGAGACTGTTTCAC | ||
| SMARCA4 | F: TGTGAGGAGGAGGAGGAGAA | 164 bp |
| R: CGCTTCCGTGATGATTTCTT |