Fig. 5.

Quantification and analysis of the mito-piR-7,456,245 derived from mitochondria in 3T3-L1 cells. a Expression of 7 selected piRNAs in NGS libraries. The heat map colors correspond to log₂ of the normalized read counts. b The RT-qPCR expression of 7 selected piRNAs in arbitrary units calculated using U6 derived piRNA as an endogenous standard. c Confocal images of cultured 3T3-L1 cells transfected with AntipiRNA-F fluorescent GapmeR, and stained with MitoTracker (mitochondria) and DAPI (nucleus). d Merged image of 3T3-L1 cells transfected with AntipiRNA-F fluorescent GapmeR, and stained with MitoTracker and DAPI. The arrows indicate the partial localization of the AntipiRNA-F fluorescent GapmeR and the mitochondria. Male cells: primordial germ cells (PGCs), spermatogonia cells (SPG), and spermatozoa (SPZ); female cells: oocytes (OCY); and zygotes (ZYGO). Bars represent 75 μm