Fig. 1.

MiR-320 was significantly upregulated in mouse myocardial tissues after I/R injury and H9c2 cells subjected to H/R. a TTC staining was used to evaluate the myocardial infarct size. Red area, area at risk, AAR; white area, infarcted tissues. The ratio of IA (infarct area) and AAR (area at risk) was calculated and presented in the bar graph. b TUNEL assay was performed to evaluate the myocardial apoptosis (scale bar = 20 μm). The relative expression levels of miR-320 were evaluated via quantitative real-time PCR (qRT-PCR) in myocardial tissues under I/R treatment and the sham group (c); cardiomyocytes under H/R and normal condition (d). Each bar or point represents the mean of three independent experiments. Error bars represent SEM. *P < 0.05