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Primer sequence of the marker genes
| Gene name | Upstream primers | Downstream primers |
|---|---|---|
| GAPDH | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| PAX7 | GAGTATAAGAGGGAGAACCCCG | TTGATTCTGAGCACTCGGCTAA |
| MyoD | GCTCTGATGGCATGATGGATTAC | CTATGCTGGACAGGCAGTCG |
| MyoG | ACTACCTTCCTGTCCACCTTCA | AGGCCTCATTCACTTTCTTGAG |
| Mrf4 | ACAGCTACAAACCCAAGCAAGA | CTTGCTCCTCCTTCCTTAGCAG |
| Myf5 | TCTGATGGCATGCCTGAATGTAA | AAGGAGCTCTTATCTGAAGCACA |
