Fig. 1.

Enzyme kinetic features of CpGPI.

A) Illustrations of the reaction catalyzed by GPI and the G6PDH-coupled assay for detecting the CpGPI enzyme activity; B) SDS-PAGE gel image showing the expression and purification of recombinant CpGPI as an MBP-fusion protein. Lane M, protein markers; Lanes 1 and 2, total proteins from transformed bacteria before and after the induction of expression by isopropyl β-D-1-thiogalactopyranoside (IPTG); Lane 3, recombinant MBP-CpGPI protein purified by amylose resin-based affinity chromatography; C) Effect of pH on the activity of CpGPI; and D) Michaelis-Menten kinetics of recombinant CpGPI towards fructose-6P. Each dataset shown here represents one of the typical experiments performed independently at least three times. Bars represent standard errors of the mean (SEMs) derived from at least three replicated reactions.