Fig. 2.

Identification of ebselen as an inhibitor of CpGPI from existing drugs.

A) Screening of the Prestwick chemical library containing 1200 existing drugs at 20 μM (open or solid round circles). Erythrose-4P at 200 μM was used as a positive control (open triangles). The three top hits are labeled by names, including the chemical structure for ebselen. B) Dose-dependent inhibition of CpGPI activity by the positive control erythrose-4P. C) Hit validation assay showing that ebselen was an authentic hit because it was ineffective on G6PDH used in the CpGPI/G6PDH-coupled assay, whereas luteolin was a false hit because its inhibition observed in the CpGPI/G6PDH-coupled assay was mainly attributed by inhibition on G6PDH. Bars represent standard errors of the mean (SEMs) derived from at least three replicated reactions.