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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Methods. 2018 Mar 31;146:39–45. doi: 10.1016/j.ymeth.2018.03.013

Figure 3.

Figure 3.

(a) Elution profiles and (b) natural logarithm of the elution profiles obtained following the application of 20 μL of 0.05 or 0.20 mg/mL labeled goat IgG onto a 5 mm × 2.1 mm I.D. protein G microcolumn. These elution profiles were obtained using a pH 2.5 elution buffer that was passed through the microcolumn at 0.50 mL/min.