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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Methods. 2018 Mar 31;146:39–45. doi: 10.1016/j.ymeth.2018.03.013

Figure 4.

Figure 4.

Apparent dissociation rate constants measured by the peak decay method for rabbit IgG as a function of flow rate. These results were obtained using a 5 mm × 2.1 mm I.D. protein G microcolumn and a pH 2.5 elution buffer that was passed through the microcolumn at 0.05–2.0 mL/min.