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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Methods. 2018 Mar 31;146:39–45. doi: 10.1016/j.ymeth.2018.03.013

Table 1.

Dissociation rate constants measured for various types of IgG with protein G at several flow ratesa

Type of IgG Flow rate and apparent dissociation rate constant, kd (s−1)
0.50 mL/min 1.0 mL/min 2.0 mL/min
Rabbit 0.125 (± 0.004) 0.220(± 0.002) 0.292 (± 0.005)
Goat 0.120 (± 0.002) 0.188(± 0.002) 0.291 (± 0.003)
Human 0.166 (± 0.001) 0.268(± 0.005) 0.374 (± 0.012)
Mouse 0.247 (± 0.003) 0.305(± 0.004) 0.452 (± 0.009)
a

The kd values were measured at pH 2.5 and room temperature. The values in parentheses represent ± 1 S.D.