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. 2001 Nov 20;98(24):13913–13918. doi: 10.1073/pnas.251532298

Figure 1.

Figure 1

rAAV-CMV-IL-4, rAAV-CMV-IL-10, and rAAV-CB-IL-10 constructs and expression in mouse myoblasts. (A) Vector cassette map where ITR, rAAV inverted terminal repeat, CMVp, CMV immediate early promoter, and CBp, CMV enhancer and chicken-β actin promoter with a hybrid chicken–rabbit β-globin intron. The circle after the gene is the simian virus 40 poly(A) signal. (B) The concentrations of IL-4 and -10 48 h after plasmid (pCMV–green fluorescent protein, pCMV-IL-4, pCMV-IL-10) transfection of C2C12 cells (performed in triplicate). (C and D) The concentrations of (C) IL-4 and (D) IL-10 0–3 days after viral (rAAV-CMV-IL-4, rAAV-CB-IL-10) transduction of C2C12 cells (performed in triplicate). Transductions with rAAV alone (□, multiplicity of infection 2,000) or under coinfection with rAAV (○, multiplicity of infection 2,000) and Ad5 (multiplicity of infection 5).