Fig. 3.

Developed HTS accurately identifies wells treated with an inhibitor of S. neurona growth. S. neurona + GFP/FLUC parasites infecting BT cells (2.0 × 10⁴ merozoites per well) in three 96-well plates were randomly treated with either 10 μM pyrimethamine (n = 12 per plate, red) or 0.1% DMSO carrier control (n = 60 per plate, blue). Uninfected BT cells were included as a negative control (n = 24 per plate, black). Infected and uninfected cells were incubated at 37 °C and 5% CO₂ for 4 days before measuring FLUC activity. The sample measurements (i.e., 96 wells per plate) for each biological replicate are separated by vertical dashed lines. The Z′ score for each replicate was calculated using FLUC activity of DMSO-treated parasites (horizontal dashed lines, 3 S.D. below normalized untreated mean) and uninfected BT cells (equation (2)). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)