Table 1.
Percentage of amino acid identity and similarity of TMD1-TMD2 domains of various Pgps relative to Cel-Pgp-1, as given by BlastP. The TMDs were extracted according to the multiple sequence alignments generated by Muscle, as shown in Suppl. Fig. S2. For each Pgp, TMD1 and TMD2 were determined by alignment with Cel-Pgp-1 TMDs as given by the 4F4C crystal structure (Jin et al., 2012). TMD1 was considered as starting at TM1 of 4F4C, thus excluding the TMa-b transmembrane hairpin not described in other known ABC transporter structures. Each TMD homology was calculated independently, and the mean of the two values is reported. Bold: Cel-Pgps and Hco-Pgps showing a long N-terminal sequence (>70 AAs). The list is displayed in decreasing order of similarity. ND: not determined as Cel-Pgp-10 presents a longer TMD1 than other Pgps.
| Cel-Pgp-1 | % Identity | % Similarity |
|---|---|---|
| Hco-Pgp-1 | 63 | 79 |
| Hco-Pgp-9.1 | 50 | 69 |
| Cel-Pgp-9 | 48 | 67 |
| Hsa-Pgp | 36 | 58 |
| Cel-Pgp-2 | 35 | 57 |
| Mmu-Abcb1a | 35 | 56 |
| Hco-Pgp-2 | 34 | 56 |
| Hco-Pgp-13 | 33 | 54 |
| Hco-Pgp-16 | 32 | 52 |
| Cel-Pgp-3 | 30 | 52 |
| Hco-Pgp-3 | 31 | 51 |
| Cel-Pgp-4 | 30 | 51 |
| Cel-Pgp-8 | 29 | 51 |
| Cel-Pgp-12 | 28 | 51 |
| Hco-Pgp-17 | 25 | 51 |
| Cel-Pgp-5 | 30 | 50 |
| Cel-Pgp-14 | 29 | 50 |
| Cel-Pgp-13 | 27 | 50 |
| Cel-Pgp-7 | 29 | 49 |
| Cel-Pgp-6a | 29 | 49 |
| Hco-Pgp-11 | 25 | 46 |
| Cel-Pgp-11 | 22 | 45 |
| Hco-Pgp-10 | 22 | 42 |
| Cel-Pgp-10 | ND | ND |