Fig. 4.

The stimuli released from co-cultured BV2 cells originated from NLRP3 inflammasome signaling pathway in BV2 cells. a The expression levels of NLRP3, ASC, pro-caspase-1, and cleaved caspase-1 in PC12 and bEnd3 cells in the transwell co-cultures in different groups, as measured by western blot. OGD/R treatment of transwell co-culture system was conducted at 24 h after siRNA transfection in BV2 cells. b The changes of NLRP3, ASC, and cleaved caspase-1 in PC12 cells among the different groups, as measured by western blot. c The changes of NLRP3, ASC, and cleaved caspase-1 in bEnd3 cells among the different groups, as measured by western blot. d The expression levels of IL-1β and IL-18 in PC12 cells among the different groups, as measured by RT-PCR and ELISA. e The expression levels of IL-1β and IL-18 in bEnd3 cells among the different groups, as measured by RT-PCR and ELISA. *p < 0.05, **p < 0.01. OGD/R: oxygen-glucose deprivation/reoxygenation. The OGD continued for 4 h, followed by reoxygenation