Figure 2.

Inhibition of HCV NS2/3 processing by inhibitors of HSP90 function. (A) ³⁵S-methionine-labeled NS2/3 (810–1615BK) was synthesized in reticulocyte lysate in the presence of either 1 or 10 μM geldanamycin, herbimycin A, or radicicol. After blocking further synthesis with cycloheximide, an aliquot was removed and processing was initiated with the addition of Triton X-100 to 1%. After 30 min, the reaction was terminated with SDS sample buffer and analysis was performed as described in Materials and Methods. (B) Each bar in the graph gives quantification of the NS2 product band in the gel lane directly above it in A Upper, expressed as percent processed relative to the control.