Figure 3.

Physical association of NS2/3 with HSP90. (A) Coimmunoprecipitation of NS2/3 with HSP90-specific antibody. ³⁵S-methionine-labeled NS2/3 (810–1615BK), Ubi-849-1207J-BLA, or firefly luciferase synthesized in RRL were immunoprecipitated with anti-HSP90 mAb 3G3 or the control IgM TEPC-183 (19). (B) Geldanamycin interferes with the association of NS2/3 and HSP90. ³⁵S-methionine-labeled NS2/3 synthesized in RRL in the absence (lane 1) or presence (lane 4) of 10 μM geldanamycin was immunoprecipitated with the anti-HSP90 mAb 3G3 (lanes 3 and 6) or the control IgM TEPC-183 (lanes 2 and 5).