Fig. 12.

Modulation in relative expression level of Sirt1 in gastrocnemius and heart muscles of exercised and BCAAs supplemented mice. qRT-PCR on cDNA samples of gastrocnemius muscle (a) and heart (b) tissues derived from exercised and 20 mg/mL BCAAs supplemented mice (20BCAA/Ex) indicated a significant increase of Sirt1 transcript level (p < 0.05). Data are shown as mean ± SEM (Standard Error of Mean) for each group (n = 6, ●). + indicates statistically significant difference with Sedentary group at p < 0.05, * indicates statistically significant difference with 20BCAA/Ex group at p < 0.05, # indicates statistically significant difference with 60BCAA/Ex group at p < 0.05, & indicates statistically significant difference with Ex group at p < 0.05, $ indicates statistically significant difference with 20BCAA group at p < 0.05, ^ indicates statistically significant difference with 60BCAA group at p < 0.05. Data of qRT-PCR were obtained on triplicate data sets for each sample and analyzed by two-way analysis of variance (ANOVA) and tukey’s post hoc test