Fig. 5.

Fndc5 transcript levels were increased in heart and muscle tissues of exercised and BCAAs supplemented mice. As elucidated, qRT-PCR on cDNA samples of gastrocnemius muscle (a) and heart (b) tissues derived from exercised and BCAAs supplemented mice indicated a significant increase in Fndc5 transcript level (p < 0.05). Data are shown as mean ± SEM (Standard Error of Mean) for each group (n = 6, ●). + indicates statistically significant difference with Sed group at p < 0.05, * indicates statistically significant difference with 20BCAA/Ex group at p < 0.05, # indicates statistically significant difference with 60BCAA/Ex group at p < 0.05, & indicates statistically significant difference with Ex group at p < 0.05, $ indicates statistically significant difference with 20BCAA group at p < 0.05, ^ indicates statistically significant difference with 60BCAA group at p < 0.05. Data of qRT-PCR were obtained on triplicate data sets for each sample and analyzed by two-way analysis of variance (ANOVA) and tukey’s post hoc test