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. 2018 Aug 29;13(8):e0201787. doi: 10.1371/journal.pone.0201787

Fig 4. HeT-A RNA and HeT-A Gag form RNPs in early embryos.

Fig 4

(A) Endogenous HeT-A RNA FISH (green) and anti-HeT-A Gag immunostaining (red) was performed on 0-2-hour old embryos after spnE_GLKD. Syncytial metaphase stage is shown. (B) HeT-A Gag (green) and gamma-tubulin (red) immunostaining was performed on 0-2-hour old embryos upon spnE_GLKD. Syncytial anaphase is shown. (C) RT-qPCR analysis of RNA precipitated with anti-HA from lysates of 0-2-hour old embryos laid by nosGal4; UAS-HeT-A-HA; UAS-spnE_sh flies relative to control nosGal4; UAS-spnE_sh embryos. log2 of fold changes (log2_FC) for RNA enrichments in RNA IP from embryos of HeT-A-HA transgenic strain versus control are shown. rp49 was used for normalization. The error bars represent SD of 2 biological replicas. Western blot analysis of immunoprecipitated HeT-A Gag-HA is shown to the right. The antibodies used for Western blotting are indicated to the right.