Fig 5. HeT-A RNPs associate with Egl in early embryos.
(A) Western blot analysis of proteins immunoprecipitated with anti-HA from 0-2-hour old embryos laid by spnE_GLKD flies with and lacking the UAS-HeT-A-HA transgene. Anti-HA immunoprecipitates HeT-A Gag-HA and Egl proteins. The antibodies used for Western blotting are indicated to the right and the antibodies used for co-IP are indicated above the IP lanes. (B) 0-2-hour old embryos of y1 w67c23 strain were immunostained with anti-Egl (red). Telophase stage of syncytial preblastoderm embryo is shown. (C) Immunostaining of 0-2-hour old embryos collected from spnE_GLKD flies was performed with anti/Gag HeT-A (green) and anti/Egl (red) antibodies. DNA is stained with DAPI (blue). Telophase is shown. Endogenous HeT-A Gag colocalizes with Egl around spindle poles (arrows).