Table 1.
Enzymes in T. aromatica or G. metallireducens a | Annotation from IMG in D. anilini | Gene locus (H567DRAFT_) b | Mol. weight (kDa) | Sequence coverage (%) c | Score d | Identities (%) e |
---|---|---|---|---|---|---|
ppsα | pyruvate, water dikinase | 02052 | 70.027 | 49.1 | 323.31 | 42 |
ppsβ | pyruvate, water dikinase | 02051 | 39.928 | 21.2 | 93.396 | 38 |
ppsγ | acetoin utilization protein AcuB | 03126 | 25.893 | 35.4 | 202.27 | 25 |
ppcβ | phenylphosphate carboxylase beta subunit | 02059 | 52.24 | 68.2 | 323.31 | 49 |
ppcδ | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (KDO 8-P phosphatase) | 00862 | – | – | – | 46 |
ppcα | phenylphosphate carboxylase beta subunit | 03563 | 51.922 | 12.9 | 76.233 | 33 |
ORF 14 | phenylacetate--CoA ligase | 02049 | 50.501 | 9 | 68.567 | 39 |
ORF 8 | 4-hydroxy-3-polyprenylbenzoate decarboxylase | 02056 | – | – | – | 56 |
BamQ* | 6-hydroxycyclohex-1-ene-1-carboxyl-CoA dehydrogenase | 01120 | 39.75 | 42.1 | 282.42 | 49 |
BamA* | 6-ketocyclohex-1-ene-1-carbonyl-CoA hydrolase | 01121 | 42.886 | 72.7 | 323.31 | 51 |
BamR* | cyclohexa-1,5-diene-1-carbonyl-CoA hydratase | 01122 | 28.001 | 55.7 | 323.31 | 37 |
BamB* | tungsten-dependent benzoyl-CoA reductase subunit bamB | 00366 | 75.838 | 46.1 | 323.31 | 80 |
aAbbreviations of the enzymes involved in phenol or benzoate (*) anaerobic degradation pathways in T. aromatica or G. metallireducens (*). bIMG gene locus tag from the genome of D. anilini. cSequence coverage represents the extent of peptides obtained during MS-MS identification of respective protein in the total proteome analyses. dThe protein score from an MS/MS search is derived from the ions scores. Score and sequence coverage of the peptide finger print match as indicated by the MASCOT-search engine. eThe highest identity of protein sequence in D. anilini to that in T. aromatica or G. metallireducens. -: Not found in total proteome analyses result