Fig. 5.

The change of amount and contents of CL and MLCL after EPA and KLA addition. RAW264.7 cells are stimulated by 100 μM EPA and 100 ng/ml KLA. The amount of CL (a) and MLCL (b) are quantified and and normalized via protein quantification. The CL and MLCL species difference between AA+KLA and KLA group are performed by direct deduction and shown in panel (c) and panel (d). Red represents the content difference more than 1 and 2.5% whereas green more than − 1 and − 2.5%, which is the one-fifth of the maximal difference, in CL and MLCL measurements, respectively. *p < 0.05, #p < 0.01