Fig 8.

Effect of once daily i.p. administration of liraglutide, (Lys⁸GluPAL)apelin-13 amide or pGlu(Lys⁸GluPAL)apelin-13 amide (each at 25 nmol/kg bw) on energy expenditure (A). Following 35 days treatment, mice were placed in CLAMS metabolic chambers for 24 h (12 h dark period as shown by the black bar) and energy expenditure calculated using RER with the following equation: (3.815 + 1.232 x RER) x VO₂. Average energy expenditure (B), energy expenditure in the light (C) and dark cycles (D) are also included. Values represent the mean ± S.E.M. (n = 6) where *p<0.05 and **p<0.01 is compared to high-fat fed saline treated mice and ^▽▽▽p<0.01 is compared to normal mice.