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. 2018 Aug 29;37:204. doi: 10.1186/s13046-018-0859-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Chemotherapy-induced MSCs are prone to differentiate into adipocytes and chondrocytes. a Osteogenic differentiation of MSCs derived from Ctrl+A2D and B-ALL+A2D by Alizarin Red S staining. b Statistical summary of osteoblast number per field in (a). c The osteoblastic differentiation markers (Gpnmb, Ogn and Sp7) were evaluated by qRT-PCR. d Chondrogenic differentiation of MSCs derived from Ctrl+A2D and B-ALL+A2D by Toluidine Blue staining. e Statistical summary of chondrogenic cell number per field in (d). f The chondrogenic differentiation markers (Acan and Col11a2) were evaluated by qRT-PCR. g Adipogenic differentiation of MSCs derived from Ctrl+A2D and B-ALL+A2D by Oil Red staining. h Statistical summary of adipogenic cell number per field in (g). i The chondrogenic differentiation markers (Cfd) were evaluated by qRT-PCR. All data in this figure were shown as mean ± SD from three separate experiments. * P ≤ 0.05; ** P < 0.01