Fig. 3.

Chemotherapy-induced MSCs have reduced levels of HSC-maintaining cytokines. a RNA-seq analysis of differentially expressed genes upregulated and downregulated more than twofold in MSCs of Ctrl+A2D and B-ALL+A2D. Blue dots represent down-regulated genes and red dots represent up-regulated genes. b Differentially expressed pathways. B-ALL+A2D versus Ctrl+A2D. c Enrichment genes analysis of B-ALL+A2D and Ctrl+A2D. d Heat map analysis of SCF, CXCL12, VCAM1, IL7 and ANGPT1 from RNA-seq data. e The expression of SCF, CXCL12, VCAM1, IL7 and ANGPT1 in MSCs of Ctrl+A2D and B-ALL+A2D was evaluated by qRT-PCR. f The expression levels of SCF, CXCL12, VCAM1, IL7 and ANGPT1 in MSCs of Ctrl+DNR and B-ALL+DNR was evaluated by qRT-PCR. g The expression levels of SCF, CXCL12, VCAM1 and IL7 in bone marrow of Ctrl+DNR and B-ALL+DNR was detected by cytokine array. All data in this figure were shown as mean ± SD from three separate experiments. * P ≤ 0.05; ** P < 0.01