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. 2018 Aug 29;13(8):e0202914. doi: 10.1371/journal.pone.0202914

Fig 1. Reduction of ERK1/2 phosphorylation by the adenosine receptor agonist NECA.

Fig 1

(A) NECA reduced the ERK1/2 phosphorylation in a concentration-dependent manner (EC50 5.85 nM, 95% confidence limit 2.91–11.8). (B) The reduction was time-dependent with a maximum after 30 min. (C) Only NECA as a nonselective agonist showed this effect while activation of A1 with CCPA, A2A (also A1 and A3 to a certain degree) with CGS 21680, and A3 with HEMADO showed no effect. Panel A and the Western blots in B and C show representative experiments, the columns in B and C show data from n = 7 and 5 independent experiments, respectively (* p < 0.001, significantly different from control).